Calorimetry Conference, CALCON 2014 (II)

The second day at CALCON 2014 started with Matthew Auton (Mayo Clinic of Minnesota) receiving the “Sting Sunner Memorial Award”. He presented his work on the thermodynamics properties of the domains of the von Willebrand factor, which mutations results in the so-called von Willebrand disease, the most common inherent bleeding disorder in man.

During the plenary session, Heiko Heerklotz (University of Toronto) showed interesting results of ITC experiments titrating surfactant into lipidic liposomes and lipidic solution into micelles systems. Analysis of this complex isotherms provides information to characterize the phase boundaries between micellar phase, fluid, ordered and gel-phase membranes.

Calorimetry Conference poster
The Calorimetry Conference, CALCON 2014

Joseph Emerson (Mississippi State University) presented his work in complex equilibria associated with metal ions coordination in dioxygen-activating enzymes. His group uses ITC data to understand how this enzymes discriminate between available metal ions.
The “hot topic” in the other thematic sessions and during the posters presentation continues being the study of structure and stability of DNA quadruplexes related to the human telomere sequence.
AFFINImeter presented the poster “AFFINImeter: A new tool to analyze Isothermal Titration Calorimetry experiments” where we showed how AFFINImeter opens up new possibilities for the application of the ITC technique such as:

AFFINImeter Applications to Isothermal Titration Calorimetry and other spectroscopy Techniques
Examples of new applications that can be studied with AFFINImeter



Calorimetry Conference, CALCON 2014 (I)

The 69th Calorimetry Conference (CALCON 2014) hold in Santa Fe (New Mexico, USA) opened today with Peter R. Tremaine (University of Guelph) receiving the “Hugh M. Huffman Memorial Award”. In his opening lecture he reviewed his research on modeling the behaviour of aqueous systems near the critical point; where many geological and industrial processes take place: from nuclear reactors to the origin of life.
In the plenary session Thomas Record (University of Wisconsin) discussed the effect of the Hofmeister series salts in the interaction with biopolymers and Randy Black (Quantum Design Inc) presented a new calorimetry cell for their measurement system to measure heats exchanges at mK temperature.
Micheael Henzl (University of Misouri) opened the “Macromolecule-Ligand Interaction” session presenting the study of the effect of mutations of Parvalbumin on its interaction with different ions. Parvalbumin is a vertebrate-specific protein believed to function primarily as cytosolic Ca2+ buffers. He showed global analysis of different ITC isotherms using a competitive binding model for Ca2+ and Mg2+ , when both ions are loaded in the syringe of the ITC equipment.

Calorimetry Conference, CALCON 2014

Cocaine-binding DNA aptamers have been developed for use in biosensor applications as they can be selected to bind almost any target with high specificity and affinity. Philip Johnson (York University) showed ITC competitive experiments between cocaine and quinine to explain the mechanism of binding with this DNA apatmers.
During the “Acid Folding and Interactions” session, different students from the Lewis Biophysics Laboratory (Mississippi State University) presented their works on the interaction of G-quadruplex DNA, one of the promising anti-cancer targets, with different ligands. They showed complex ITC isotherms with two inflexion points which they analysed with a binding model that takes into account three different events: the binding of the first ligand, the unfolding of the G-quadruplex DNA structure and the binding of a second ligand.
Tomorrow AFFINImeter will be in the poster session presenting the poster “AFFINImeter: A new tool to analyze Isothermal Titration Calorimetry experiments”. On Wednesday we will present an oral communication on the same topic during the “Global Analysis, Modeling and Simulations” session.

CALCON 2014, 69th Calorimetry Conference

Dr. Juan Sabin, a member of our scientific department, will participate with an oral presentation at the 69th Calorimetry Conference (Santa Fe, NM 2014) to describe the main features of AFFINImeter like the “model builder”, an easy-to-use tool with which researchers will design their own binding models, the “global fitting” of a series of isotherms or the analytical tool to explore possible “local minima” generated from the fitting procedure.

He will show how AFFINImeter opens up new possibilities for the application of the ITC technique such as:

  • Thermodynamic Characterization of ligand induced conformational changes.
  • Competitive binding models with high order complexes.
  • Quantitative analysis discrimination of ligands in heterogeneous mixtures (i.e. isomers, heteropolymers…).
  • Accurate analysis of ultra low-affinity or ultra high affinity complexes.
  • Characterization of binding processes with no restriction in the stoichiometry.
  • Micellization studies to obtain the aggregation number, enthalpy and Gibbs energies of micelle formation.
  • Global simultaneous analysis of isotherms (i.e. global analysis of curves from direct and reverse experiments).

Don’t miss the talk at /


20th International Symposium on Surfactants in Solution (Coimbra, Portugal)

The AFFINImeter innovation team has developed an original model to analyze dissociation ITC isotherms of aggregates ranging from (protein) dimers to large supramolecular clusters (like micelles).  With this model the average number of molecules in the aggregates, the molar free energy and enthalpy of transfer from the aggregate to the solution and the dilution of both monomers and aggregates can be obtained. This model soon will be available in the AFFINImeter Software.

The validation of this model comparing our results with those determined from other experimental methods is going to be presented in the 20th International Symposium on Surfactants in Solution (SIS, June 2014), where our CSO Angel Piñeiro is giving Oral presentation on Monday  June 23, 2014.  

We won the 4th Poster Award in DiPIA 2014


AFFINImeter attended the Development in Protein Interaction Analysis (DiPIA) Conference in La Jolla, CA this June 2014, where we were part of the leading scientists working in protein interactions.

We are glad to tell you that our Poster of AFFINImeter: a new tool for analysis of Isothermal Titration Calorimetry experiments has been awarded with the 4th prize of the poster competition. Thanks to our representative J. Sabín of doing such a good job presenting AFFINImeter.

If you haven’t registered yet to try the beta version visit our webpage or contact us at

Download the poster here

AFFINImeter is present in the DiPIA 2014 Conference

This weekend we are joining the leading scientists at the Development in Protein Interaction Analysis (DiPIA) conference. A representative of AFFINImeter will present  a new tool to analyze Isothermal Titration Calorimetry Experiments.

New generations of Isothermal Titration Calorimetry (ITC) equipments have significantly improved their performance in terms of higher sensitivity and lower sample size requirements. Thus, physicochemical processes that were undetectable in the past by ITC are now sensed with high precision. In contrast to the progress achieved in instrumentation, ITC data analysis is still limited in many aspects. Researchers often face complicated situations that cannot be represented by standard binding models i.e. the formation of higher order complexes, the presence of multiple candidates to bind the host molecule and/or the simultaneous occurrence of binding and aggregation processes.

AFFINImeter allows to design personalized binding models directly in chemical language, through an original user-friendly interface that allows the design of advanced models.


AFFINImeter Applications to Isothermal Titration Calorimetry and other spectroscopy Techniques
Examples of new applications that can be studied with AFFINImeter