AFFINImeter is on sales!

 

This month we have prepared a special offer just to let you exploit the full potential of AFFINImeter:

 15 % off in our Advanced plans*.

Select the technique AFFINImeter Spectroscopy and/or AFFINImeter ITC.

 

          

 

… Use this coupon in our online shop and get right away your discount!

ER7DS30

To use our online shop it will be necessary to have an AFFINImeter account, to create one click here.

AFFINImeter Shop

Or if you prefer we can send you an official quotation, click on the button below:​

Get a Quotation

 

 

*The promotion is available until May 31st and applicable for all AFFINImeter Advanced plan (ITC and/or Spectroscopy) which duration is equal or greater 1 year. The advanced account includes Global fitting, access to complex binding models, Kinetic information (KinITC tool available for AFFINImeter ITC), orthogonal approach (Multitechnique analysis available in AFFINImeter Spectroscopy)...

 

 

 

What does it look like to you?

It might look like a plain hat or an elephant being eaten by a boa constrictor, but actually, the shape of this curve looks like a typical spectrum of UV-Vis determined by changes in the intensity of this energy and also it represents the logo of our new software:

AFFINImeter for spectroscopic techniques!

 

Hence, we want to take this opportunity to launch AFFINImeter Spectroscopy software.

It is a new software for the processing and advanced analysis of binding isotherms obtained from different spectroscopic techniques: 1D-NMR, Uv-vis, fluorescence, circular dichroism, differential scanning fluorimetry…

 

…and from now on, you will be able to start using it:

Get a free trial


To get started, we have prepared some scientific material as an example of how to use AFFINImeter Spectroscopy:


We have prepared a special offer to celebrate the launch of AFFINImeter for Spectroscopic techniques.
Using this code coupon you will get a 25% off*, to buy through our online shop:

Spectroscopy_coupon


 
 
 You can also ask for a quotation here 
 
 
*The discount will be applied until 2018/03/03.

Global fitting: the key for a robust analysis

Download use case: Global Fitting

 

The Indian parable of “The six blind men and the elephant” tells the story of six blind men who touch an elephant in the hope of learning what it is like. As each one can only feel a different part of the animal the individual conclusions obtained are in disagreement and none of them provides a real view of the full elephant. “only by sharing what each of you knows can you possibly reach a true understanding”; that´s the moral behind this nice story.


 

Fig 1: The six blind men and the elephant: only a global analysis of the overall data provides a true understanding.

The binding assay(s) achieved to characterize a molecular interaction often provides not just one, but several binding curves from which the affinity constant is obtained.
Sometimes, an individual fit of these curves yield a set of binding constants that are significantly different from them; this result can be very confusing because, in principle, these binding curves are a representation of the same binding event and should converge to provide the analogous information. Often, the explanation for this behaviour is that the different curves indeed provide only partial and/or different information of the interaction, not enough to unequivocally determine the binding affinity through individual analysis.


“It´s like feeling only a separate part of the elephant”


This is a typical scenario when facing the study of complex binding events that involve more than one equilibrium and several binding curves are obtained, i.e. from different frequencies of the spectra in a titration experiment, from data registered using different techniques (ITC, NMR, Optical Spectroscopies…) and/or from experiments performed at different concentrations of the species participating in the binding event.


Analogous to the parable of the six men and the elephant, the way to get a true understanding of the binding event consist of the global analysis of the different curves.

Fig 2: The binding curve obtained from 2D NMR titrations.


Being aware of the relevance of global analysis, in AFFINImeter we count with the possibility to perform Global fitting of multiple data to tailored binding models where one or more fitting parameters are shared between isotherms. The number and identity of the parameters shared are selected by the user.
Moreover, two or more parameters can be related through mathematical relationships designed by the user. All these features make our global fitting tool the most potent among others to perform a robust analysis of binding data of complex interactions.