Tag: Calorimetry Conference

5 Tips to optimize your ITC experiments for kinetic analysis.

Eva Muñoz

Since the method KinITC was implemented in AFFINImeter many researchers have been using it to obtain kinetic information of binding interactions from ITC data; the good news is that no special experimental setup different from the standard ITC experiment is required to register data for kinetic analysis! The information is derived from analysis of the thermogram of regular ITC titrations and therefore one can obtain kinetic information from old ITC data right away.

There are few recommendations though if you are planning to perform new ITC experiments, focused on getting high-quality data for kinetic analysis:

1) Set the time between successive power measurements to 1s or 2s. This will give a better definition of the thermogram peaks and therefore a more precise calculation of the equilibration times.

2) Set the time recording the baseline before the first injection to 1 or 2 minutes. In order to have a good reference when determining the signal baseline.

3) Leave enough time between injections so that a full equilibration for the overall set of injections is registered.

4) Clean thoroughly the instrument before the experiment. This is fundamental to optimize the response time of the instrument, which strongly determines the sensitivity of the kinetic analysis.

5) A high gain feedback mode is recommended in order provide the fastest response time (but, be careful because a high feedback mode can also generate signal overshooting after injection, which greatly difficulties the kinetic analysis! If overshooting happens, don´t use high gain model).

Need more information about this subject? Contact the Scientific team of AFFINImeter at info@affinimeter.com.

Follow these simple tips to increase the quality of your ITC data for kinetic analysis

Figure Junio2016

AFFINImeter and Isothermal Titration Calorimetry

AFFINImeter

AFFINImeter and Isothermal Titration Calorimetry: A perfect match for the complete Thermodynamic and Kinetic Characterization of molecular interactions

  1. Get thermodynamic and Kinetic profiles of your interaction in a few clicks.
  2. Get structural and mechanistic information of complex interacting systems.
  3. Quick access to Data Interpretation Tools.

Have a look at poster of AFFINImeter presented at the NovAlix conference last week, which describes the main features of AFFINImeter with illustrative examples. Many researchers put special attention to the fast and efficient way of processing raw ITC data to get, in just 5 clicks, thermodynamic and kinetic information. Check the video below for a quick demo of this awesome tool!

 

Get the kinetic parameters from standard ITC experiments in 5 clicks.

AFFINImeter & Isothermal Titration Calorimetry from AFFINImeter

CALCON 2014, 69th Calorimetry Conference

AFFINImeter

Dr. Juan Sabin, a member of our scientific department, will participate with an oral presentation at the 69th Calorimetry Conference (Santa Fe, NM 2014) to describe the main features of AFFINImeter like the “model builder”, an easy-to-use tool with which researchers will design their own binding models, the “global fitting” of a series of isotherms or the analytical tool to explore possible “local minima” generated from the fitting procedure.

He will show how AFFINImeter opens up new possibilities for the application of the ITC technique such as:

  • Thermodynamic Characterization of ligand induced conformational changes.
  • Competitive binding models with high order complexes.
  • Quantitative analysis discrimination of ligands in heterogeneous mixtures (i.e. isomers, heteropolymers…).
  • Accurate analysis of ultra low-affinity or ultra high affinity complexes.
  • Characterization of binding processes with no restriction in the stoichiometry.
  • Micellization studies to obtain the aggregation number, enthalpy and Gibbs energies of micelle formation.
  • Global simultaneous analysis of isotherms (i.e. global analysis of curves from direct and reverse experiments).

Don’t miss the talk at http://www.calorimetry-conference.org /