Reliability of the thermodynamic parameters obtained from Isothermal Titration Calorimetry experiments

How to obtain thermodynamic parameters?


Binding Reaction Scheme
Protein-Ligand Binding Scheme

Isothermal Titration Calorimeters have been designed to determine the thermodynamic parameters corresponding to physicochemical processes that take place in a solution: protein-ligand binding, enzyme assay, drug encapsulation, dissociation/aggregation, micellization, etcThe reliability of the thermodynamic parameters obtained from Isothermal titration calorimetric experiments depends critically on:

(i) the volume injected in the sample cell upon each titration

(ii) the concentration of the solution in the sample cell

(iii) the concentration of the solution in the injection system

(iv) the number of titrations

(v) the number and relative concentration of the different chemical species that can be formed upon mixing of both solutions

(vi) the heat involved in the formation of those chemical species

(vii) the order in which the solutions are considered, i.e., which solution is introduced into the sample cell and which is in the injection system.