The course of Isothermal Titration Calorimetry data analysis: second part

In the second part of this course, we are going to show you how to perform the analysis of binding isotherms considering an independent sites approach.
This approach uses a reaction scheme based on the binding of the ligand to individual sites present in the receptor and considering that all the sites are independent; thus, it supplies site binding constants.
This approach offers a sole reaction scheme where a receptor with a certain number of sites “n” binds to the ligand.
The sites are grouped into sets to discern between sites that are non-equivalent.

If you want to know more about how to get the stoichiometry (number of sites) and site binding constants with the independent sites approach you can click here:

Stoichiometric and site constants – two approaches to analyze data with AFFINImeter

The first video tutorial presented is about how to use an independent sites approach to perform fittings:


Into another subject, to introduce the second fitting approach that can be performed with AFFINImeter (Stoichiometric equilibria), we will describe how to use the model builder.
This original tool allows to design and apply your own binding model in an easy way. Check the following video to know the versatility of the model builder:

Finally, if you want to try the Model Builder click here:

Model Builder


Tecnologías de vanguardia en biomedicina: Orbitrap (ion trap mass analyzer) y SPR (Surface Plasmon Resonance

La Dr. Eva Muñoz, miembro del staff científico de S4SD ha sido invitada para participar en el seminario  “Tecnologías de vanguardia en biomedicina: Orbitrap (ion trap mass analyzer) y SPR (Surface Plasmon Resonance)” que tendrá lugar el día 16 de Diciembre en Vigo. El seminario es organizado por el Instituto de Investigación Biomédica de Ourense-Pontevedra-Vigo, en el marco del proyecto BIOCAPS

Al seminario también  están invitados la Dr. Mónica Carrera (Instituto de Investigaciones Marinas de Vigo, IIM-CSIC) experta en proteómica, el Dr. Manuel Marcos (Universidad de Vigo, CACTI) para hablar de aplicaciones de  Orbitrap (Ion trap mass analyzer) y la Dra. Pilar Canoa (Universidad de Vigo, CINBIO) para hablar de aplicaciones de SPR (Surface plasmon resonance).

La inscripción es gratuita y está abierta hasta el día 14 de Diciembre de 2015.

AFFINImeter guest presenter of the Malvern webinar on advanced Isothermal Titration Calorimetry (ITC) data analysis

Webinar on advanced Isothermal Titration Calorimetry (ITC) data analysis

The AFFINImeter team are guest presenters of the Malvern webinar on advanced Isothermal Titration Calorimetry (ITC) data analysis. Together we will show how the latest advances in the field of ITC data analysis enable users to “squeeze” the ITC isotherm(s) to get more information than just thermodynamic data and to expand the range of applications of ITC. This webinar will be very helpful for ITC users studying complex interactions, for those who are having difficulties with their data analysis, or simply want to take their analysis to the next level. It is of special interest relevant for researchers from academia and industry working in biomedical applications, particularly in the area of drug design.


“Molecular Recognition: new tools for complex situations”

June 24, 2014 Spanish National Research Council (CSIC)

Conference póster Eva Muñoz
“Molecular Recognition: new tools for complex situations”

Dr. Eva Muñoz, a member of our scientific department, was invited to the Centre for Organic Chemistry Manuel Lora Tamayo (CSIC) in Madrid where she gave a talk entitled “Molecular Recognition: new tools for complex situations”. During the talk she described various examples of complex interacting systems difficult to evaluate with standard analytical methods, and the use of new experimental and analytical approaches to circumvent these problems. Here, AFFINImeter was presented as a novel analytical tool for the analysis of complex ITC data.
Moreover, she introduced AFFINImeter in a seminar at the Biological Research Centre, CIB (CSIC, Madrid) explaining how it can be a must tool in the molecular recognition research field. She described the main features of the application like the “model builder”, an easy-to-use tool with which researchers design their own binding models, the “global fitting” of a series of isotherms or the analytical tool to explore possible “local minima” generated from the fitting procedure.