Recently we received the great news because AFFINImeter has been used and mentioned in some scientific publications.
The first one, It´s a review of the applications of Isothermal Titration Calorimetry from 2011 to 2015 . The author describes that:
“AFFINImeter produces commercial software that can be used for analysis of displacement assays, micellisation experiments, kinITC, the application of complex models for complex interactions and ligand-induced conformational change.” R. J. Falconer
Press on the links bellow to take a look at the publications:
Since the method KinITC was implemented in AFFINImeter many researchers have been using it to obtain kinetic information of binding interactions from ITC data; the good news is that no special experimental setup different from the standard ITC experiment is required to register data for kinetic analysis! The information is derived from analysis of the thermogram of regular ITC titrations and therefore one can obtain kinetic information from old ITC data right away.
There are few recommendations though if you are planning to perform new ITC experiments, focused on getting high-quality data for kinetic analysis:
1) Set the time between successive power measurements to 1s or 2s. This will give a better definition of the thermogram peaks and therefore a more precise calculation of the equilibration times.
2) Set the time recording the baseline before the first injection to 1 or 2 minutes. In order to have a good reference when determining the signal baseline.
3) Leave enough time between injections so that a full equilibration for the overall set of injections is registered.
4) Clean thoroughly the instrument before the experiment. This is fundamental to optimize the response time of the instrument, which strongly determines the sensitivity of the kinetic analysis.
5) A high gain feedback mode is recommended in order provide the fastest response time (but, be careful because a high feedback mode can also generate signal overshooting after injection, which greatly difficulties the kinetic analysis! If overshooting happens, don´t use high gain model).
Need more information about this subject? Contact the Scientific team of AFFINImeter at email@example.com.
Follow these simple tips to increase the quality of your ITC data for kinetic analysis
During the last months we’ve contacted you asking your opinion and experience with the software. Thanks to all your suggestions and comments we have improved the previous version of the software to make it easier, faster and more versatile.
What’s new in AFFINImeter?
- Availability of KinITC for TA and Microcal data files.
- Inclusion of Multi Temperature Analysis: Van’t Hoff and Eyring plots
- The project management section has improved, now you can easily organize your projects in Folders/Subfolders and move them from one to another.
Easier, Faster and more Versatile!
In this new version you will find several changes adressed to improve the user experience of the software, adding more features, making it easier and faster to user, and more versatile.
Go to the software!
La Dr. Eva Muñoz, miembro del staff científico de S4SD ha sido invitada para participar en el seminario “Tecnologías de vanguardia en biomedicina: Orbitrap (ion trap mass analyzer) y SPR (Surface Plasmon Resonance)” que tendrá lugar el día 16 de Diciembre en Vigo. El seminario es organizado por el Instituto de Investigación Biomédica de Ourense-Pontevedra-Vigo, en el marco del proyecto BIOCAPS.
Al seminario también están invitados la Dr. Mónica Carrera (Instituto de Investigaciones Marinas de Vigo, IIM-CSIC) experta en proteómica, el Dr. Manuel Marcos (Universidad de Vigo, CACTI) para hablar de aplicaciones de Orbitrap (Ion trap mass analyzer) y la Dra. Pilar Canoa (Universidad de Vigo, CINBIO) para hablar de aplicaciones de SPR (Surface plasmon resonance).
La inscripción es gratuita y está abierta hasta el día 14 de Diciembre de 2015.
As a launching promotion 10 FREE hrs of data analysis consulting are included into your advanced plan.
- This advanced plan includes unlimited technical support relative to the use of AFFINImeter.
- 10 hours of data analysis consulting relative to your particular interaction system and data. Each hr is valuated in 120€ and during this launching promotion these are for free.
- Remember that the current Free trial promotion includes 2 hrs data analysis consulting.
AFFINImeter and Isothermal Titration Calorimetry: A perfect match for the complete Thermodynamic and Kinetic Characterization of molecular interactions
- Get thermodynamic and Kinetic profiles of your interaction in a few clicks.
- Get structural and mechanistic information of complex interacting systems.
- Quick access to Data Interpretation Tools.
Have a look at poster of AFFINImeter presented at the NovAlix conference last week, which describes the main features of AFFINImeter with illustrative examples. Many researchers put special attention to the fast and efficient way of processing raw ITC data to get, in just 5 clicks, thermodynamic and kinetic information. Check the video below for a quick demo of this awesome tool!
Get the kinetic parameters from standard ITC experiments in 5 clicks.
NovAliX Conference 2015
AFFINImeter will be at the 2nd NovAliX conference that will be held next June, days 9-12, in Strasbourg, France.
Our Scientific team will present the poster
AFFINImeter & ITC: A PERFECT MATCH FOR THE COMPLETE THERMODYNAMIC AND KINETIC CHARACTERIZATION OF MOLECULAR INTERACTIONS:
The use of Biophysical methods for the characterization of molecular interactions has become a fundamental milestone in the area of pharmaceutical research. Into this subject, Isothermal Titration Calorimetry, ITC, is considered the gold standard for the thermodynamic characterization of molecular interactions, but with a potential that has not been fully exploited yet.
How many experiments have you carried out in your Isothermal Titration Calorimeter during the last years? Do you know that you can reuse them to obtain kinetic information?
KinITC is a methodology recently developed by Philippe Dumas (CNRS, France) to simultaneously get kinetic and thermodynamic information from a standard ITC experiment. The current implementation of kinITC in AFFINImeter is valid only for 1:1 interactions but we intend to extend this for more complex systems in the near future.
If you have performed experiments of 1:1 interactions in the past, bring them back and use KinITC to get kinetic constants (kon and koff). Under ideal conditions (perfectly clean sample cell, accurate characterization of the response time of your instrument and sufficiently slow kinetics) the resulting values are expected to be equivalent to those obtained by surface plasmon resonance (SPR). The whole process takes just a few seconds and you can analyse multiple files simultaneously.
Blow the dust off your old ITC binding experiments! Go to AFFINImeter!
(1) Burnouf D1, Ennifar E, Guedich S, Puffer B, Hoffmann G, Bec G, Disdier F, Baltzinger M, Dumas P. kinITC: a new method for obtaining joint thermodynamic and kinetic data by isothermal titration calorimetry. J. Am. Chem. Soc. 2012 Jan 11; 134(1):559-65. doi: 10.1021/ja209057d. Epub 2011 Dec 16.
The AFFINImeter team are guest presenters of the Malvern webinar on advanced Isothermal Titration Calorimetry (ITC) data analysis. Together we will show how the latest advances in the field of ITC data analysis enable users to “squeeze” the ITC isotherm(s) to get more information than just thermodynamic data and to expand the range of applications of ITC. This webinar will be very helpful for ITC users studying complex interactions, for those who are having difficulties with their data analysis, or simply want to take their analysis to the next level. It is of special interest relevant for researchers from academia and industry working in biomedical applications, particularly in the area of drug design.